Consulta: subjectFacets:"Real-time PCR analysis"
Registros recuperados: 2
Data/hora: 19/05/2024 04:07:43

Differential expression of metallothionein type-2 homologues in leaves and roots of Black pepper (Piper nigrum L)
Provedor de dados: Genetics and Molecular Biology
Autores: Alex,Susan M.; Dicto,Jose; Purushothama,M.G.; Manjula,S.
Black pepper (Piper nigrum L.), member of the family Piperaceae is indigenous to India and is one of the most widely used spices in the world. In this paper we report the results of our attempts to identify a set of genes differentially expressed in the leaves of Piper nigrum, which could facilitate targeted engineering of this valuable crop. A PCR-based Suppression Subtractive Hybridization (SSH) technique was used to generate a leaf-specific subtracted cDNA library of Piper nigrum. A tester population of leaf cDNA was subtracted with a root derived driver cDNA. The efficiency of subtraction was confirmed by PCR analysis using the housekeeping gene actin. On sequence analysis, almost 30% of the clones showed homology to metallothionein type-2 gene. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Piper nigrum; Suppression subtractive hybridization; Metallothionein type-2; Real-time PCR analysis; Northern blotting.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300024

Enhanced levels of the apoptotic BAX/BCL-2 ratio in children with acute lymphoblastic leukemia and high-risk features
Provedor de dados: Genetics and Molecular Biology
Autores: Kaparou,Maria; Choumerianou,Despoina; Perdikogianni,Chrysoula; Martimianaki,Georgia; Kalmanti,Maria; Stiakaki,Eftichia.
It has been suggested that leukemia is characterized by an impaired balance between the proliferation of blood cells and their capacity to undergo apoptosis. The aim of this study was to examine the expression of key molecules related to apoptosis (BCL-2, BAX, FAS, FAS-L) in children with acute lymphoblastic leukemia (ALL). Measurement of BCL-2 and BAX mRNA was performed by quantitative real-time PCR, and membrane expression of FAS and FAS-L was assessed by flow cytometry in bone marrow mononuclear cells, both at diagnosis and at remission following induction chemotherapy. At diagnosis, increased levels of the apoptotic BAX/BCL-2 ratio were observed in children older than 10 years and with higher white blood cell counts. A DNA index &lt; 1.16 was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukemia; Real-time PCR analysis; Protein.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100002